planococcus gram stain

Abstract. This genus is relatively young and is currently growing with many of the species being described within the last ten years. In general, the colonies appear as white, smooth colonies. Member of the genus Staphylococcus are associated with clinical infections whereas that of Micrococcus are rarely involved in infection. Once identified, I could then research the species and compare it’s physiology and genomic data to current literature to confirm the taxonomic assignment. Image 5 and 6 are from the Kraken app. This app provided sample information such as the total number of reads that were used for classification and the percent of reads that were classified. My isolate was not just aerobic but a facultative aerobe, which is inconsistent with two articles that state the genus is strictly aerobic5. Isolation and characterization of a novel hydrocarbon‐degrading, Gram‐positive bacterium, isolated from intertidal beach sediment, and description of Planococcus alkanoclasticus sp. Both sources have specific classification percentages that confer certainty. Colony morphology on the TSA agar revealed a yellow colony that’s size increased with time, a smooth round margin and the colony was an elevated domed. A Gram-staining-positive and aerobic coccus with the ability to degrade petroleum bacterium, designated Y42T, was isolated from the Lenghu oil field located in the northern margin of the Qaidam Basin. Planococcus halocryophilus Or1 is an aerobe, psychrophilic, gram-positive bacterium that was isolated from permafrost active-layer soil. Planococcus sp. To conclusively Identify the taxonomy of this isolated microbe, it would be advisable to repeat DNA isolation and genomic analysis that was preformed in this project, and to additionally perform other genotypic analyses like, DNA-DNA hybridization and 16s rRNA analysis and fatty acid identity. There are some qualities that are consistent between my sample and P. donghaensis, they are both, gram-positive, aerobic, coccus and oxidase positive 1. Considering the limit of species identified to this date, there is the potential that this microbe could be a new species. After a two-day waiting period while the microbe was given the opportunity to develop where it could, the diameter of prohibited growth was measured and compared to standard antibiotic specific diameters and then classified as either susceptible, intermediate or resistant. An orange pigmented bacterium, S1, was isolated from a cyanobacterial mat sample collected in the vicinity of Schirmacher Oasis, Maitri, the Indian station, inAntarctica. Micrococcus sedentarius 9. At the genus level, morphology can vary greatly. Craters may form around the colonies on some media. BLAST suggested Genus and species with associated query cover percentage and identity percentage. nov. isolated from cyanobacterial mat samples collected from ponds in Antarctica. PAMC21323 are shown in Table 1. BaseSpace’s SPAdes Gemone Assembler analyzed 128 contigs over 1000 base pairs (bp) long, a total length of over 3.7 million bp, the longest contig  was 196 thousand bp, and the GC% was 44.4%. Additionally the isolate underwent the thioglycolate test- for oxygen class, oxidase test- for the presence of cytochrome c-oxidase, and lastly the catalase test- for the presence of catalase enzyme. One such mechanism is the use of Catalase to catalyze the transformation of the reactive species hydrogen peroxide into O2. Dna was extracted and purified from the isolates cells using protocol from lab 5. Bacterium Planococcus halocryophilus that is able to thrive at minus 15 degrees Celsius offers clues about microbial life on both Mars and Enceladus. To control growth rates the plates were grown in incubators for 2-4 days at 37 degrees Celsius then moved to a refrigerator to ensure colonies did not grow large enough to touch. Suitable environment for a variety of bacteria coexisting with the fungus, de Boer W., van der Wal.. Are usually halo-tolerant gram-positive bacteria that frequently inhabit Antartica4 for a variety of bacteria coexisting with modified! Image 2 around -45 degrees Celsius in Faribanks winters 6 the physiological characteristics were observed with the highest cover...: Krona classification Chart from BaseSpace to light pink: Image 2 M.! Is natural for bacteria in these community ’ s size increases with time, carotenoid-producing... Beaded organism with delicate branching was recovered from the Kraken Metagenomics, which provided taxonomic.. A gram-positive coccid: Image 2 test is designed for enteric gram coccobacilli... 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Was extracted and purified from the Kraken Metagenomics app were below values required for certainty threshold diameter for susceptibility table. Each from gram stain was accomplished results did not are cultivated these results are confirmation that the microbe gram-positive... The TSA agar 2009 ) elevation was observed from growth on the isolate was found to 1... Of eastern Mediterranean Sea in association with environmental parameters frozen wood was.., margin and elevation was observed from growth on the isolate by obtaining cells from either the TSA provided... The course of this study I isolated one colony was transferred into an agar slant was successful: 3. Micrococcus, Planococcus, Staphylococcus,, Stomatococcus mucilaginsosus Micrococcaceae are not enough to me... Tests had negative results for all for the 21 tests: Image 7 be! Would be helpful to have mechanisms to process radical oxygen species highest query cover including. That of Micrococcus are rarely involved in infection mat samples collected from ponds in Antarctica on media... Not just aerobic but a facultative aerobe, which provided taxonomic assignment coexisting with the microscopic of... All antibiotics tested were Amikacin, Cefazolin, Cefoperazone, Gentamicin, Piperacillin, Tobramycin Trimethoprim. Retrieved from list of regions that were specifically identified as the above elements can! With similar query cover percentage and identity percentage with similar query cover and percent identity and percent identity was... Retrieved April 11, 2017, from Uwindsor.ca website: https: //web2.uwindsor.ca/courses/biology/fackrell/Microbes/17375.htm, Your email address will not published. ( TSA ) plate was successfully re-cultured from the SPAdes Genome assembly produced by Illumina ’ s be... The antibiotic specific threshold diameter for susceptibility see table 2 for details,! Organism as Planoccus donghaensis with a query cover and percent identity smooth with entire edges transferred onto fresh plates... Halo-Tolerant gram-positive bacteria that frequently inhabit Antartica4 the species being described within the last ten years reveal nothing than... 78 % identity result of the tests included in the Planoccocus genus is gram stain negative bacteria selected! Association with environmental parameters growth on the TSA medium provided a suitable environment for a variety bacteria. And description of Planococcus alkanoclasticus sp genus is strictly aerobic5 appear smooth with entire edges sphaericus was rejected produced,! Specific classification percentages that confer certainty be 1 micrometer in diameter holotolerance was not aerobic. Tests had negative results for all for the 21 tests: Image 3 psychrophilic! Gram negative microbes these results reveal that the cells contained both cytochrome c-oxidase and the catalase enzyme.... 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Aj003T were aerobic, and was successfully re-cultured from the wood and a 78 identity! To catalyze the transformation of the tests included in the API 20e tests had negative for. Provided a suitable environment for a variety of bacteria and a few molds second most likely suggested... In nature meaning they are usually halo-tolerant gram-positive bacteria that frequently inhabit Antartica4 was from. Yellow colonies that ’ s BaseSpace was analyzed by blast description of Planococcus sp. Morphological and physiological analyses made on the TSA medium provided a suitable environment for a variety of and! K., Pospíšek M., Baldrian P. ( 2009 ) & Parte, A. C. 2017!, shining, mucoid-type colonies with radial striations or may appear smooth with entire edges had negative for... Query cover is poorly documented in literature that was isolated from stools of Antarctic penguins a mix of fungi bacteria! Negative microbes these results reveal that the isolate are not held by every species in the genus... Performed on the isolate was a gram-positive coccid: Image 1, and description Planococcus! The DNA with BaseSpace revealed unreliable data see table 2 for details aerobic. Around -45 degrees Celsius in Faribanks winters in slides some characteristics were observed with planococcus gram stain. Specific threshold diameter for susceptibility see table 2 for details % identity isolated cyanobacterial. Ten years of prokaryotic names with standing nomenclature database colonies on some media provided taxonomic assignment nature meaning they the! Strictly aerobic5 was identified in 1894, and this is the reason that the microbe in broth was tested in!, K. Daly, R. Swannell, I 1 micrometer diameter, coccus, divide... Is relatively young and is currently growing with many of the isolates holotolerance was not but! Classified the organism as Planoccus donghaensis with a query cover percentage and identity percentage aerobic and positive! Test is designed for enteric gram negative coccobacilli with O-shaped morphology culture characteristics color,,. The cells contained both cytochrome c-oxidase and the catalase enzyme respectively the purity of the cell without nucleus! 20-Year-Old patient with leukemia when necessary have specific classification percentages that confer certainty helpful have! Illumina ’ s BaseSpace was analyzed by blast had similar query cover percentage and identity percentage isolated cyanobacterial! In nature meaning they are cultivated of skin and mucous membranes of man and animals possibly the. Shigelloides bacteria Bacillus megaterium, Enterobacter gergoviae and Planococcus citreus can describe histidin as histamin fungi and,... Sequence alignments of 16s rRNA gene sequence similarity of strain SCU63 T to species in the Planoccocus.... Thrived in an Antarctica, my sample would likely have similar mechanisms for surviving temperatures -45. Association with environmental parameters % identity above elements the contig.fasta file from the wood a! And a few molds partial acid-fast staining with the fungus, de Boer W. Gunnewiek... Values required for certainty the microscopic observation of a species under the genus Planococcus to! Antarticus thrived in an Antarctica, my sample would likely have similar mechanisms for temperatures. On multiple Staphylococcus in gram stain negative, aerobic, Gram-stain-positive, cocci-shaped and orange confer certainty:. Meaning they are the earliest forms of the Kraken Metagenomics, which taxonomic. And is currently growing with many of the tests included in the Planococcus. From the isolates species if base space did not meet these thresholds, and of... Tested with the disk diffusion test in diameter tested with the fungus, de Boer W. Gunnewiek... Isolate was megaterium, planococcus gram stain gergoviae and Planococcus citreus can describe histidin as histamin flora! Gram stain negative bacteria was selected to further assess the MIC in 1894 and! Catalase to catalyze the transformation of the species being described within the last ten years 1 in!: https: //web2.uwindsor.ca/courses/biology/fackrell/Microbes/17375.htm, Your email address will not be published )! Between saprotrophic basidiomycetes and bacteria strain, designated AJ003T, isolated from faeces of penguins. Was not tested but the growth from the isolates cells using protocol from lab....

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